Labile, poorly expressed, or poorly soluble proteins paose many difficulties in isolation and purification. These difficulties can be overcome, however, by various means. One approach is to modify ...

Bed-height Optimization Can Reduce Cost and Increase Speed of Production Affinity chromatography is potentially the most selective method for protein purification and is often included as a primary ...

Affinity chromatography can be applied to a wide array of proteins and works by exploiting the binding properties between proteins and ligands. It involves passing a recombinant protein mixture over a ...

The present purification protocol applies to target proteins that are fused to a double tag, such as NusA–His 6, through a linker that includes a protease-recognition sequence. It involves two steps ...

Previous purification methods, such as ammonium sulphate precipitation, were known to yield a crude purification of proteins. Affinity purification of antibodies is one of widely used techniques where ...

Antibodies are essential proteins produced by the immune system that recognize and bind to specific targets, known as antigens. Their high specificity and affinity make antibodies invaluable tools in ...